Regulation of bovine corneal endothelial cell cycle by transforming growth factor-beta
| Autor: | Yutaka, Motegi, Tomohiro, Usui, Kouichi, Ishida, Satoshi, Kato, Hidetoshi, Yamashita |
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| Rok vydání: | 2003 |
| Předmět: |
Cell Cycle
Endothelium Corneal Endoglin G1 Phase Vascular Cell Adhesion Molecule-1 Receptors Cell Surface Proto-Oncogene Proteins c-sis Resting Phase Cell Cycle S Phase Transforming Growth Factor beta1 Transforming Growth Factor beta2 Antigens CD Transforming Growth Factor beta Animals Humans Cattle Receptors Transforming Growth Factor beta Cells Cultured Protein Kinase C Signal Transduction |
| Zdroj: | Acta ophthalmologica Scandinavica. 81(5) |
| ISSN: | 1395-3907 |
| Popis: | The transforming growth factor-beta (TGF-beta) family includes three multifunctional proteins, TGF-beta1, TGF-beta2 and TGF-beta3, expressed in ocular tissue, which are involved in regulating cell differentiation, cell proliferation and other cell functions. TGF-beta is present in aqueous humour and regulates corneal endothelial cells. This study explores the mechanism by which TGF-beta regulates the cell cycle in cultured corneal endothelial cells.The expression of specific receptors for the TGF-beta family was investigated at the protein level by affinity cross-linking with radio-iodinated TGF-beta1 and immunoprecipitation with specific antibodies to TGF-beta receptors. Regulation of entry into the S-phase of the cell cycle was determined by 5-bromo-2' deoxyuridine (BrdU) incorporation into the cells. The signal transduction pathways were investigated using various blocking agents for protein kinase transducers involved in intracytoplasmic signal transduction.Cultured bovine corneal endothelial cells were confirmed to express TGF-beta type 1 and type 2 receptors and endoglin. In the confluent state, TGF-beta1 and TGF-beta2 stimulated the cells to progress to the S-phase of the cell cycle through platelet-derived growth factor-B (PDGF-B) chain production and protein kinase C.TGF-beta accelerated cell cycle progression from the G0/G1 phase to the S-phase in cultured corneal endothelial cells, under our experimental conditions, through pathways involving protein kinase C. These pathways are related to the cross-talk between TGF-beta and other cytokines. The conditions employed in the present experiments may be useful for investigating the complex cross-talk between various cytokines and growth factors. |
| Databáze: | OpenAIRE |
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