In vivo expression and regulation of murine IL 2 receptors after antigen sensitization
| Autor: | L D, Butler, P E, DeRiso, P, Marder, M E, Scheetz |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Immunosuppression Therapy
Time Factors T-Lymphocytes Cyclosporins Mice Inbred Strains Receptors Interleukin-2 Picryl Chloride Flow Cytometry Lymphocyte Activation Dexamethasone Mice Histocompatibility Antigens Immune Tolerance Animals Interleukin-2 Immunization Lymph Nodes Receptors Immunologic Cyclophosphamide |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 138(2) |
| ISSN: | 0022-1767 |
| Popis: | We have studied the in vivo regulation and expression of the murine IL 2 receptor after antigen sensitization. High and low affinity receptor expression has been studied by flow cytometry analysis and radiolabeled IL 2 binding. Both anti-IL 2 receptor antibody and unlabeled IL 2 inhibited the radiolabeled IL 2 binding. The kinetics of expression of the IL 2 receptor closely correspond to the proliferative response, as assessed by IUdR radioisotope uptake. The expression of IL 2 receptors correlated with the proliferative response profile of the murine strains surveyed during a study of responses to picryl chloride. Neither immune compromised 4-mo-old MRL/lpr mice nor athymic nude mice generated significant antigen-specific proliferative responses or IL 2 receptor expression after antigen sensitization. Mice rendered specifically unresponsive to antigen displayed reduced proliferative responses to the tolerizing antigen, as well as reduced numbers of IL 2 receptor-positive cells. Finally, the treatment of mice with immunosuppressive drugs reduced both the proliferative responses as well as the number of IL 2 receptor-positive cells. However, at the cellular level, the drugs produced different effects on IL 2 receptor expression. |
| Databáze: | OpenAIRE |
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