| Autor: |
B, Brossat, J, Straczek, M H, Heulin, X, Herbeuval, F, Belleville, P, Nabet, F, Lokiec |
| Rok vydání: |
1987 |
| Předmět: |
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| Zdroj: |
Journal of chromatography. 413 |
| Popis: |
A simple reliable liquid chromatographic method for assay of cyclosporin A in serum or urine is described. Samples were cleaned up on a solid-phase extraction system (cyanopropyl column). The system involved a reversed-phase C18 Ultrasphere column maintained at 72 degrees C and an acetonitrile linear gradient (65 to 95%) in 0.14% triethylammonium phosphate. Liquid chromatographic analysis of radioimmunoassay standards shows that some samples contain a contaminant peak. Comparison of cyclosporin A levels obtained by radioimmunoassay and high-performance liquid chromatography in clinical investigations show that the former values are generally, but not always, higher than the latter, and that cyclosporin A is very differently metabolized depending on the patient, disease and treatment. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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