Detection of bovine meat and bone meal in animal feed at a level of 0.1%

Autor:	Henk J M, Aarts, El M, Bouw, Jaap B, Buntjer, Johannes A, Lenstra, Leo W D, Van Raamsdonk
Rok vydání:	2007
Předmět:	Meat Sheep Reverse Transcriptase Polymerase Chain Reaction Equipment Reuse Animals Cattle DNA Animal Feed Chickens Bone and Bones
Zdroj:	Journal of AOAC International. 89(6)
ISSN:	1060-3271
Popis:	For the control of the transmission of bovine spongiform encephalopathy in cattle via feedstuff, a real-time polymerase chain reaction assay was developed with ruminant-specific Bov-B SINE primers, SYBR Green fluorescence detection, and melting curve analysis. In formulated cattle and chicken feed samples spiked with pure bovine and sheep meat and bone meal heated at 133 degrees C for 20 min, a contamination level of 0.1% was detected.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::34840b00d85d038f0d12c1e29cda617b https://pubmed.ncbi.nlm.nih.gov/17225589 Zobrazit plný text záznamu