| Autor: |
Maud, de Lagarde, Caroline, Larrieu, Karine, Praud, Nathalie, Lallier, Angélina, Trotereau, Guillaume, Sallé, John M, Fairbrother, Catherine, Schouler, Benoît, Doublet |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
International journal of antimicrobial agents. 55(6) |
| ISSN: |
1872-7913 |
| Popis: |
The objective of the study was to identify the genetic determinants and characteristics of expanded-spectrum cephalosporin (ESC) resistance in commensal Escherichia coli from healthy horses in France in 2015. Faecal samples from 744 adult horses were screened for ESC-resistant E. coli isolates. The extended-spectrum beta-lactamase (ESBL)/AmpC resistance genes were identified using polymerase chain reaction (PCR) and sequencing. ESC phenotypes were horizontally transferred by conjugation or transformation. Plasmids carrying ESBL/AmpC genes were typed by PCR-based replicon typing, restriction fragment length polymorphism (RFLP), and plasmid multilocus sequence typing (pMLST). The ESC-resistant E. coli isolates were typed by XbaI macrorestriction analysis. Sixteen of 41 stables harboured at least one horse carrying ESC-resistant E. coli. The proportion of individually tested horses carrying ESC-resistant E. coli was 8.5% (28/328). Fifty non-redundant ESC-resistant E. coli isolates showing a great diversity of XbaI macrorestriction profiles belonged mainly to phylogroup B1, and were negative for major E. coli virulence genes, indicating they are commensal isolates. ESBL bla |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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