Targeting mammary epithelial cells using a bacterial artificial chromosome

Autor:	Tim M, Wintermantel, Anja K, Mayer, Günther, Schütz, Erich F, Greiner
Rok vydání:	2002
Předmět:	Chromosomes Artificial Bacterial Integrases Genetic Vectors Gene Expression Epithelial Cells Mice Transgenic Milk Proteins Cell Line Mice Viral Proteins Organ Specificity Gene Targeting Animals Breast
Zdroj:	Genesis (New York, N.Y. : 2000). 33(3)
ISSN:	1526-954X
Popis:	We describe the generation of transgenic mouse lines expressing Cre recombinase in epithelial cells of the lactating mammary gland. As an expression vector, we used a P1-derived bacterial artificial chromosome (PAC) which harbors the gene for the secretory milk protein, whey acidic protein (Wap). Using homologous recombination in E. coli, the PAC was modified to carry the improved coding sequence of Cre recombinase (iCre). Transgenic lines carrying the WAPiCre PAC express Cre recombinase efficiently in the majority of mammary epithelial cells upon lactation. Of only four transgenic lines produced, three express Cre recombinase to a high efficiency. LoxP-flanked DNA sequences are recombined in virtually all epithelial cells of WAPiCre transgenic mice at lactation day 3.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::344b6370ff3124f7304af30579f815a6 https://pubmed.ncbi.nlm.nih.gov/12124945 Zobrazit plný text záznamu Plný text