| Autor: |
Sarah J, Bourlat, Quiterie, Haenel, Jennie, Finnman, Matthieu, Leray |
| Rok vydání: |
2016 |
| Předmět: |
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| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 1452 |
| ISSN: |
1940-6029 |
| Popis: |
This protocol details the preparation of multiplexed amplicon libraries for metabarcoding (amplicon-based) studies of microscopic marine eukaryotes. Metabarcoding studies, based on the amplification of a taxonomically informative marker from a collection of organisms or an environmental sample, can be performed to analyze biodiversity patterns or predator-prey interactions. For Metazoa, we use the mitochondrial cytochrome oxidase 1 (CO1) or the small ribosomal subunit (SSU) markers. Here, we describe a strategy for the preparation of multiplexed Illumina MiSeq libraries using a dual-PCR approach for the addition of index and adaptor sequences. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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