Infectious disease modeling and innate immune function in zebrafish embryos
| Autor: | Chao, Cui, Erica L, Benard, Zakia, Kanwal, Oliver W, Stockhammer, Michiel, van der Vaart, Anna, Zakrzewska, Herman P, Spaink, Annemarie H, Meijer |
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| Rok vydání: | 2011 |
| Předmět: |
Salmonella typhimurium
Salmonella Infections Animal Embryo Nonmammalian Microinjections Neutrophils Macrophages Genomics Flow Cytometry Immunity Innate High-Throughput Screening Assays Molecular Imaging Gene Knockdown Techniques Animals Humans Cell Lineage Transgenes Biomarkers In Situ Hybridization Fluorescence Zebrafish Developmental Biology |
| Zdroj: | Methods in cell biology. 105 |
| ISSN: | 0091-679X |
| Popis: | The major cell types of the innate immune system, macrophages and neutrophils, develop during the first two days of zebrafish embryogenesis. The interaction of these immune cells with pathogenic microbes can excellently be traced in the optically transparent zebrafish embryos. Various tools and methods have recently been developed for visualizing and isolating the zebrafish embryonic innate immune cells, for establishing infections by different micro-injection techniques, and for analyzing the host innate immune response following microbial recognition. Here we provide practical guidelines for the application of these methodologies and review the current state of the art in zebrafish infectious disease research. |
| Databáze: | OpenAIRE |
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