| Autor: |
Yue-Hua, Zhang, Nan-Ping, Wang, Qi-Ping, Zhang, Xiao-Qiu, Shi |
| Rok vydání: |
2011 |
| Předmět: |
|
| Zdroj: |
Yao xue xue bao = Acta pharmaceutica Sinica. 44(12) |
| ISSN: |
0513-4870 |
| Popis: |
The fluorescence spectroscopy and UV spectroscopy have been used to monitor the inclusion phenomena of VP-16 with beta-cyclodextrin (beta-CD), together with studies concerning the effects of reaction time, temperature and concentration on this behavior. The results show that the fluorescence intensity increased when VP-16 and beta-CD forming a 1 : 1 inclusion complex, which indicate that beta-CD has fluorescence sensitizing effect on the VP-16. At 22 degrees C, the inclusion constant was 2.63 x 10(5) L x mol(-1) at pH 7.0. VP-16 has maximum emission wavelength at 316 nm under the optimum conditions. According to this, the quantitative micro-detection method of VP-16 by fluorescence spectrometry was established. The linear regression equation was y = 1.107 89 x 10(70 x + 95.898 1, with a correlation coefficient of 0.999 9. The detection limit was 2.094 x 10(-7) mol x L(-1). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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