Introduction of a proximal Stat5 site in the murine alpha-lactalbumin promoter induces prolactin dependency in vitro and improves expression frequency in vivo
| Autor: | S, Soulier, L, Lepourry, M G, Stinnakre, B, Langley, P J, L'Huillier, J, Paly, J, Djiane, J C, Mercier, J L, Vilotte |
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| Rok vydání: | 1999 |
| Předmět: |
Binding Sites
Transcription Genetic Gene Expression Regulation Developmental Gestational Age Mice Transgenic CHO Cells Milk Proteins Transfection Prolactin DNA-Binding Proteins Mice Inbred C57BL Mice Organ Specificity Pregnancy Cricetinae Lactalbumin Mice Inbred CBA STAT5 Transcription Factor Trans-Activators Animals Female Promoter Regions Genetic |
| Zdroj: | Transgenic research. 8(1) |
| ISSN: | 0962-8819 |
| Popis: | In order to establish a possible correlation between in vitro prolactin induction and the transcriptional activity of mammary gene promoters in transgenic mice, a functional Stat5-binding site was created by means of site-directed mutagenesis at position -70 on a 560 bp murine alpha-lactalbumin promotor linked to a CAT reporter gene. Surprisingly, the wild-type promoter was constitutively active in vitro and could not be induced by prolactin. Introducing the proximal Stat5 site abolished this constitutive activity and resulted in prolactin dependence in both CHO-K1- and HC11-transfected cells. In transgenic mice, both the frequency of lines expressing the transgene and the prevalence of mid to late pregnancy expression were increased. |
| Databáze: | OpenAIRE |
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