Differential transcription of two highly divergent gut-expressed Bm86 antigen gene homologues in the tick Rhipicephalus appendiculatus (Acari: Ixodida)
Autor: | L, Kamau, R A, Skilton, D O, Odongo, S, Mwaura, N, Githaka, E, Kanduma, M, Obura, E, Kabiru, A, Orago, A, Musoke, R P, Bishop |
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Rok vydání: | 2010 |
Předmět: |
Male
Vaccines DNA Complementary Membrane Glycoproteins Base Sequence Reverse Transcriptase Polymerase Chain Reaction Molecular Sequence Data Gene Expression Recombinant Proteins Gastrointestinal Tract Rhipicephalus Animals Female Amino Acid Sequence Cloning Molecular 3' Untranslated Regions Sequence Alignment |
Zdroj: | Insect molecular biology. 20(1) |
ISSN: | 1365-2583 |
Popis: | The transcriptional control of gene expression is not well documented in the Arthropoda. We describe transcriptional analysis of two exceptionally divergent homologues (Ra86) of the Bm86 gut antigen from Rhipicephalus appendiculatus. Bm86 forms the basis of a commercial vaccine for the control of Rhipicephalus (Boophilus) microplus. The R. appendiculatus Ra86 proteins contain 654 and 693 amino acids, with only 80% amino acid sequence identity. Reverse-transcription PCR of gut cDNA showed transcription of only one genotype in individual female ticks. PCR amplification of 3' untranslated sequences from genomic DNA indicated that both variants could be encoded within a single genome. When both variants were present, one of the two Ra86 genotypes was transcriptionally dominant. |
Databáze: | OpenAIRE |
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