[Blocking p38MAPK pathway inhibits the proliferation of OT-II cells mediated by splenic dendritic cells]
| Autor: | Ling, Han, Yuechen, Luo, Wantong, Wu, Yongfei, Wang, Sanmei, Ma |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
CD4-Positive T-Lymphocytes
Lipopolysaccharides MAP Kinase Signaling System Pyridines Receptors Antigen T-Cell Enzyme-Linked Immunosorbent Assay Mice Transgenic p38 Mitogen-Activated Protein Kinases Animals Enzyme Inhibitors Cells Cultured Cell Proliferation Antigen Presentation Histocompatibility Antigens Class II Imidazoles Dendritic Cells Flow Cytometry Coculture Techniques Peptide Fragments Mice Inbred C57BL B7-1 Antigen Cytokines Female B7-2 Antigen Inflammation Mediators Spleen |
| Zdroj: | Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 32(3) |
| ISSN: | 1007-8738 |
| Popis: | To investigate the effect of blocking p38 mitogen-activated protein kinase (p38MAPK) pathway on the proliferation of OT-II cells mediated by splenic dendritic cells (DCs).Splenic DCs of C57BL/6 mice were purified with anti-CD11c immunomagnetic beads, and OT-II cells were isolated from the splenic of CD4(+)-ovalbumin transgenic mice (OT-IItransgenic mice) by mouse CD4 T cell isolation kits. After being pretreated with SB203580, an inhibitor of p38MAPK, DCs were stimulated with lipopolysaccharides (LPS). Then the expression levels of co-stimulatory molecules (CD80, CD86) and MHCII in DCs, and antigen-presenting ability of DCs treated with the 52-68 fragment of the E alpha-chain of I-E class II molecules (Eα52-68 peptide) were detected by flow cytometry. The protein levels of tumor necrosis factor α (TNF-α), interleukin 1α (IL-1α), interleukin 6 (IL-6) and transforming growth factor β (TGF-β) in the culture supernatants were measured by ELISA. The proliferation of OT-II cells which were co-cultured with OVA323-339-treated DCs was analyzed by flow cytometry.The purity of both DCs and OT-II cells reached over 90% after isolation. SB203580 downregulated the expressions of CD80, CD86 and MHC II, and suppressed the antigen-presenting ability of DCs. The expressions of TNF-α, IL-1α and IL-6 were downregulated, while the expression of TGF-β was raised. Finally, SB203580 inhibited DCs-mediated proliferation of OT-II cells.Blocking p38MAPK pathway with SB203580 could inhibit DCs-mediated proliferation of OT-II cells, which might be involved in modulating the expressions of CD80, CD86 and MHC II, the antigen-presenting ability, as well as the expressions of pro-inflammatory and anti-inflammatory cytokines. |
| Databáze: | OpenAIRE |
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