| Autor: |
J O, Boison, C D, Salisbury, W, Chan, J D, MacNeil |
| Rok vydání: |
1991 |
| Předmět: |
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| Zdroj: |
Journal - Association of Official Analytical Chemists. 74(3) |
| ISSN: |
0004-5756 |
| Popis: |
An improved method has been developed for the determination of benzyl penicillin in animal tissues. Tissues are fortified with a known amount of penicillin V (internal standard) and extracted with water. The extract is deproteinized with sulfuric acid and sodium tungstate, filtered, and concentrated on a conditioned C18 solid phase extraction column. Penicillin V and benzyl penicillin are then eluted from the column with 1 mL 60% acetonitrile-35% water-5% 0.2M phosphate buffer solution and derivatized with 1 mL 1,2,4-triazole-mercuric chloride solution at 65 degrees C for 30 min. An aliquot of this sample is analyzed by reverse phase liquid chromatography with UV detection at 325 nm. The limit of detection is 5 micrograms/kg (ppb) penicillin G (8.4 IU/kg) in liver, kidney, and muscle tissues). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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