Integrins beta 5, beta 3 and alpha v are apically distributed in endometrial epithelium
| Autor: | J D, Aplin, C, Spanswick, F, Behzad, S J, Kimber, L, Vićovac |
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| Rok vydání: | 1996 |
| Předmět: |
Integrins
Integrin beta Chains Ovariectomy Integrin beta3 Antibodies Monoclonal Platelet Membrane Glycoproteins In Vitro Techniques Integrin alphaV Polymerase Chain Reaction Epithelium Endometrium Mice Estrus Antigens CD Pregnancy Animals Humans Female Embryo Implantation RNA Messenger Fluorescent Antibody Technique Indirect |
| Zdroj: | Molecular human reproduction. 2(7) |
| ISSN: | 1360-9947 |
| Popis: | Several adhesion molecules have been shown to occur at the surface of endometrial cells. One of these is the integrin alpha v subunit which associates with various beta chains including beta 5. We demonstrate the presence of integrin beta 5 polypeptide in human endometrial epithelial cells throughout the menstrual cycle using immunocytochemistry with monospecific antibodies, and at the mRNA level by thermal amplification from endometrial cDNA. Integrin beta 5 is also found in a population of bone marrow-derived cells. A notable feature of the distribution of the beta 5 subunit in the glandular and luminal epithelium is its apical localization, which may suggest an involvement in implantation. However, no evidence was found for regulated expression of epithelial beta 5. In mouse, the beta 5 subunit is found at both the apical and basal surface of epithelial cells and expression is essentially oestrous cycle-independent. Comparisons are made in both species with the distribution of the alpha v and beta 3 subunits which also localize to the apical epithelium. |
| Databáze: | OpenAIRE |
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