Enhanced detection of HIV-1 sequences using polymerase chain reaction and a liquid hybridization technique. Application for individuals with questionable HIV-1 infection

Autor:	S M, Bruisten, M H, Koppelman, C L, van der Poel, J G, Huisman
Rok vydání:	1991
Předmět:	Acquired Immunodeficiency Syndrome Base Sequence AIDS-Related Complex Blotting Western DNA Viral HIV-1 Humans Nucleic Acid Hybridization Polymerase Chain Reaction Plasmids
Zdroj:	Vox sanguinis. 61(1)
ISSN:	0042-9007
Popis:	In this report we describe a sensitive HIV-1 detection method which is applicable for confirmation testing of donors whose blood sample gives indeterminate viral-serology results. The method involves performing a polymerase chain reaction (PCR) and detecting the generated fragments using liquid hybridization and gel retardation. We found that it is as specific as blotting on a filter and hybridization with an internal probe but at least tenfold more sensitive. After applying it on DNA samples of a panel of 11 persistent indeterminate anti-p24gag-reactive donors, none was found to be PCR positive. Considering other negative virological and biochemical test results and case-historical data, these donors are not likely to be HIV-1 infected.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::32e7cf44c6a84ea4209f4a5fb59468e2 https://pubmed.ncbi.nlm.nih.gov/1949706 Zobrazit plný text záznamu Plný text