Effect of residual acrylamide monomer from two-dimensional gels on matrix-assisted laser desorption/ionization peptide mass mapping experiments

Autor:	E J, Alvarez, B S, Larsen, C D, Coldren, J, Rice
Rok vydání:	2000
Předmět:	Acrylamides Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Electrophoresis Gel Two-Dimensional Peptide Mapping Peptide Fragments
Zdroj:	Rapid communications in mass spectrometry : RCM. 14(11)
ISSN:	0951-4198
Popis:	Residual acrylamide can cause severe suppression of signal intensity during matrix-assisted laser desorption/ionization (MALDI) peptide mass mapping experiments. This suppression phenomenon can compromise the ability to detect low picomole and subpicomolar amounts of peptides extracted from two-dimensional gels. A rapid and simple method that exploits the use of pipette tips incorporating C18 packing materials for the enhancement of MALDI signal intensity is presented. The utility of the method is demonstrated with peptide solutions incorporating residual acrylamide and/or gel monomer components.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::32e44a055bebb9a7db0b7c825d6eeac2 https://pubmed.ncbi.nlm.nih.gov/10844734 Zobrazit plný text záznamu Plný text