| Autor: |
N V, Shipulina, N Iu, Tomilina, G F, Zhirnov, V B, Lisitsyna, I E, Kovalev, A I, Archakov |
| Rok vydání: |
1989 |
| Předmět: |
|
| Zdroj: |
Biokhimiia (Moscow, Russia). 54(8) |
| ISSN: |
0320-9725 |
| Popis: |
The feasibility of obtaining a conjugated benz(a)pyrene-protein antigen in the liver cytochrome P-450 system was studied. Covalent binding of benz(a)pyrene (BP) to albumin was performed with the use of liver microsomal fractions of 3-methylcholanthrene-induced rabbits. It was demonstrated that BP oxidation in liver microsomes is accompanied by covalent binding of [14C]BP to exogenous rabbit albumin. Immunization of rabbits with the obtained conjugate results in the development of a specific immune response to BP, and the appearance of specific antibodies and lymphocytes specifically binding [14C]BP in the blood. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
