Involvement of cyclic nucleotides and IP(3) in the regulation of luminescence in the brittlestar Amphipholis squamata (Echinodermata)

Autor:	N, De Bremaeker, Y, Dewael, F, Baguet, J, Mallefet
Rok vydání:	2000
Předmět:	Luminescence Inositol 1 4 5-Trisphosphate Enzyme Activation Bucladesine Guanylate Cyclase Type C Phospholipases Adenylyl Cyclase Inhibitors Cyclic AMP Dibutyryl Cyclic GMP Animals Calcium Enzyme Inhibitors Cyclic GMP Adenylyl Cyclases Echinodermata
Zdroj:	Luminescence : the journal of biological and chemical luminescence. 15(3)
ISSN:	1522-7235
Popis:	We investigated the effects of cyclic nucleotides (cGMP, cAMP) and the phosphoinositide IP(3) on the luminescence of the ophiuroid Amphipholis squamata. The cGMP analogue, dibutyryl-cGMP, and the guanylate cyclase activator, sodium nitroprusside, had no effect on the luminescence. The cAMP analogue, dibutyryl-cAMP, and the adenylate cyclase activator, forskolin, triggered luminescence. Moreover, the adenylate cyclase inhibitor, MDL-12330A, significantly reduced ACh-induced luminescence. The phospholipase C inhibitor, U-73122, also significantly reduced ACh-induced luminescence. The results suggest that ACh-induced luminescence is mediated by both cAMP and IP(3) pathways but not by cGMP. The effects of calcium-free ASW confirmed this hypothesis. A hypothetical scheme of the transduction mechanisms involved in the intracellular control of luminescence is presented.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::326b4c476d138c4bedf748c462534218 https://pubmed.ncbi.nlm.nih.gov/10862144 Zobrazit plný text záznamu Plný text