Function of the TCR alpha enhancer in alphabeta and gammadelta T cells
| Autor: | B P, Sleckman, C G, Bardon, R, Ferrini, L, Davidson, F W, Alt |
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| Rok vydání: | 1997 |
| Předmět: |
Mice
Knockout Transcription Genetic Receptors Antigen T-Cell alpha-beta T-Lymphocytes Gene Rearrangement delta-Chain T-Cell Antigen Receptor Receptors Antigen T-Cell gamma-delta Thymus Gland Mice Inbred C57BL Mice Enhancer Elements Genetic Gene Expression Regulation Mice Inbred CBA Animals Gene Rearrangement alpha-Chain T-Cell Antigen Receptor Genes T-Cell Receptor alpha Sequence Deletion |
| Zdroj: | Immunity. 7(4) |
| ISSN: | 1074-7613 |
| Popis: | We have used gene targeted mutational approaches to assess the role of the T cell receptor alpha (TCR alpha) enhancer (E alpha) in the control of TCR alpha and TCR delta gene rearrangement and expression. We show that E alpha functions in cis to promote V alpha to J alpha rearrangement across the entire J alpha locus, a distance of greater than 70 kb. We also show that E alpha is required for normal alphabeta T cell development; in this lineage, E alpha is required for germline J alpha expression, for normal expression levels of rearranged V alpha J alpha genes, and for expression of a diverse V alpha repertoire. In gamma delta T cells, E alpha is not required for VdeltaDJdelta rearrangement, but, surprisingly, is required for normal expression levels of mature VdeltaDJdelta transcripts and for expression of germline J alpha transcripts. Our findings imply that E alpha function is not limited to the TCR alpha components of the TCRalpha/delta locus or to the alpha beta lineage; rather, E alpha function is important in both alphabeta and gammadelta lineage T cells. |
| Databáze: | OpenAIRE |
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