| Autor: |
Kutty Selva, Nandakumar, Mattias, Collin, Kaisa E, Happonen, Susanna L, Lundström, Allyson M, Croxford, Bingze, Xu, Roman A, Zubarev, Merrill J, Rowley, Anna M, Blom, Christian, Kjellman, Rikard, Holmdahl |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Frontiers in Immunology |
| ISSN: |
1664-3224 |
| Popis: |
Endo-β-N-acetylglucosaminidase (EndoS) is a family 18 glycosyl hydrolase secreted by Streptococcus pyogenes. Recombinant EndoS hydrolyzes the β-1,4-di-N-acetylchitobiose core of the N-linked complex type glycan on the asparagine 297 of the γ-chains of IgG. Here, we report that EndoS and IgG hydrolyzed by EndoS induced suppression of local immune complex (IC)-mediated arthritis. A small amount (1 µg given i.v. to a mouse) of EndoS was sufficient to inhibit IgG-mediated arthritis in mice. The presence of EndoS disturbed larger IC lattice formation both in vitro and in vivo, as visualized with anti-C3b staining. Neither complement binding in vitro nor antigen-antibody binding per se were affected. Thus, EndoS could potentially be used for treating patients with IC-mediated pathology. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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