The pharmacology of hSK1 Ca2+-activated K+ channels expressed in mammalian cell lines
| Autor: | Shah, M, Haylett, D G |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Potassium Channels
Small-Conductance Calcium-Activated Potassium Channels Xenopus Tubocurarine Nicotinic Antagonists Kidney Transfection Cell Line Rats Potassium Channels Calcium-Activated Apamin Dequalinium COS Cells Chlorocebus aethiops Potassium Channel Blockers Animals Humans Calcium Special Report |
| Popis: | The pharmacology of hSK1, a small conductance calcium-activated potassium channel, was studied in mammalian cell lines (HEK293 and COS-7). In these cell types, hSK1 forms an apamin-sensitive channel with an IC(50) for apamin of 8 nM in HEK293 cells and 12 nM in COS-7 cells. The currents in HEK293 cells were also sensitive to tubocurarine (IC(50)=23 microM), dequalinium (IC(50)=0.4 microM), and the novel dequalinium analogue, UCL1848 (IC(50)=1 nM). These results are very different from the pharmacology of hSK1 channels expressed in Xenopus oocytes and suggest the properties of the channel may depend on the expression system. Our findings also raise questions about the role of SK1 channels in generating the apamin-insensitive slow afterhyperpolarization observed in central neurones. |
| Databáze: | OpenAIRE |
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