| Autor: |
Diana, Sánchez-Palencia, Swetha, Rathan, Casey J, Ankeny, Ruth, Fogg, Juan C, Briceño, Ajit P, Yoganathan |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
Journal of tissue engineering and regenerative medicine. 11(5) |
| ISSN: |
1932-7005 |
| Popis: |
In small intestinal submucosa (SIS) scaffolds for functional tissue engineering, the impact of scaffold fabrication parameters on cellular response and tissue regeneration may relate to the mechanotransductory properties of the final arrangement of collagen fibres. We previously proved that two fabrication parameters, (a) preservation (P) or removal (R) of a dense collagen layer present in SIS, and (b) SIS in a final dehydrated (D) or hydrated (H) state, have an effect on the micromechanical environment of SIS. In a continuation of our studies, we herein hypothesized that these fabrication parameters also modulate early mechanotransduction in cells populating the scaffold. Mechanotransduction was investigated by seeding human umbilical vein endothelial cells (HUVECs) on scaffolds, exposing them to pulsatile shear stress (12 ± 4 dyne/cm |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
