Vectors for rapid selection of integrants with different plasmid copy numbers in the yeast Hansenula polymorpha DL1

Autor:	M O, Agaphonov, P M, Trushkina, J H, Sohn, E S, Choi, S K, Rhee, M D, Ter-Avanesyan
Rok vydání:	1999
Předmět:	Blotting Southern Transformation Genetic Gene Dosage Chromosomes Fungal DNA Fungal Pichia Plasmids
Zdroj:	Yeast (Chichester, England). 15(7)
ISSN:	0749-503X
Popis:	Plasmids with different selectable markers were constructed and used to transform the Hansenula polymorpha strain DL1. It was shown that, depending on the host mutant strain, the use of these plasmids enables rapid selection of transformants with plasmids integrated in low (1-2), moderate (6-9) or high (up to 100) copy numbers. The vectors and mutant described are potentially useful for the construction of efficient producers of heterologous proteins in H. polymorpha.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::31160a4d528b5f87da24227a78d4308b https://pubmed.ncbi.nlm.nih.gov/10341417 Zobrazit plný text záznamu Plný text