| Autor: |
Fang-Fang, Zhang, Ying, Cheng, Ning, Wan, Zi-Wei, Jing, Jia, Ju, Yi-Yang, Jia, Si-Yuan, Zhou, Bang-Le, Zhang |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
Biomedical chromatography : BMC. 29(9) |
| ISSN: |
1099-0801 |
| Popis: |
A rapid, sensitive HPLC-MS/MS method was established and validated to assay the concentration and pharmacokinetic profile of MT502, a promising hypnotic drug. The plasma sample was treated by a liquid-liquid extraction and separated on a kromasil C18 column at an isocratic flow rate of 0.3 mL/min using methanol and 0.1% formic acid in water (75:25, v/v) as mobile phase. The mass spectrometric detection was carried out using a triple-quadrupole system via positive electrospray ionization. Multiple reaction monitoring was used for quantitation of m/z transitions from 261 to 188 for MT502 and from 247 to 188 for MT501 (internal standard). Good linearity was achieved over the concentration range of 1-1000 ng/mL and 10-5000 ng/mL with lower limit of quantification of 0.30 and 0.80 ng/mL. The intra- and inter-day precisions, accuracy, recovery and stability were satisfactory for the concentration test. The above method can be used for a pharmacokinetic study at doses of 1, 5 and 20 mg/kg. Results indicated that MT502 had rapid absorption, rapid elimination and linear pharmacokinetic properties within the range of the tested intragastric dose. This developed HPLC-MS/MS method was successfully applied to a pharmacokinetic study of MT502 for the first time and was demonstrated to be simple and sensitive. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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