| Autor: |
G, Gamberi, M S, Benassi, T, Böhling, P, Ragazzini, L, Molendini, M R, Sollazzo, M, Merli, C, Ferrari, G, Magagnoli, F, Bertoni, P, Picci |
| Rok vydání: |
1998 |
| Předmět: |
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| Zdroj: |
Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 16(1) |
| ISSN: |
0736-0266 |
| Popis: |
Giant-cell tumor is a primary bone tumor, of uncertain origin, with the potential capacity to metastasize. To study the role of c-myc and c-fos oncogene overexpression in the tumorigenesis and metastatic spread of giant-cell tumors, 32 primary tumors were collected; of these, 19 remained disease-free and 13 metastasized to the lung. Samples of lung metastasis from these 13 patients were also available for study. The expression of c-myc and c-fos mRNA was studied by reverse transcription-polymerase chain reaction and by in situ hybridization. The expression of protein was studied by Western blot analysis and by immunohistochemistry. C-myc mRNA was overexpressed in 12 (38%) of the 32 primary tumors. Thirteen primary tumors metastasized to the lung; in nine (69%) of these, c-myc mRNA was overexpressed. The c-myc protein was overexpressed in seven (54%) of the 13 tumors that metastasized to the lung. C-fos was overexpressed in only one lung metastasis. A strong correlation between the overexpression of c-myc, and the occurrence of metastases was found: thus, c-myc seems a powerful prognosticator in giant-cell tumor. C-myc was overexpressed both in giant cells and in mononuclear cells, suggesting that both cell types are involved in the progression of this tumor. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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