| Autor: |
V S, Valentinuzzi, D E, Kolker, M H, Vitaterna, K, Shimomura, A, Whiteley, S, Low-Zeddies, F W, Turek, E A, Ferrari, R, Paylor, J S, Takahashi |
| Rok vydání: |
1999 |
| Předmět: |
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| Zdroj: |
Learningmemory (Cold Spring Harbor, N.Y.). 5(4-5) |
| ISSN: |
1072-0502 |
| Popis: |
The most commonly measured mouse behavior in fear conditioning tests is freezing. A technical limitation, particularly for genetic studies, is the method of direct observation used for quantifying this response, with the potential for bias or inconsistencies. We report the use of a computerized method based on latency between photobeam interruption measures as a reliable scoring criterion in mice. The different computer measures obtained during contextual fear conditioning tests showed high correlations with hand-scored freezing; r values ranged from 0.87 to 0.94. Previously reported strain differences between C57BL/6J and DBA/2J in context-dependent fear conditioning were also detected by the computer-based system. In addition, the use of computer-scored freezing of 199 (BALB/cJ × C57BL/6J)F2 mice enabled us to detect a suggestive gender-dependent chromosomal locus for contextual fear conditioning on distal chromosome 8 by QTL analysis. Automation of freeze scoring would significantly increase efficiency and reliability of this learning and memory test. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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