Cytogenetic damage and induction of pro-oxidant state in human lymphocytes exposed in vitro to gliphosate, vinclozolin, atrazine, and DPX-E9636

Autor:	M B, Lioi, M R, Scarfi, A, Santoro, R, Barbieri, O, Zeni, F, Salvemini, D, Di Berardino, M V, Ursini
Rok vydání:	1998
Předmět:	Chromosome Aberrations Oxidative Stress Humans Urea Atrazine Lymphocytes Glucosephosphate Dehydrogenase Pesticides Oxidants Oxazoles Sister Chromatid Exchange Cells Cultured Mutagens
Zdroj:	Environmental and molecular mutagenesis. 32(1)
ISSN:	0893-6692
Popis:	We analyzed chromosome aberrations (CAs), sister chromatid exchanges (SCEs), mitotic index (MI), and glucose 6-phosphate dehydrogenase (G6PD) enzyme activity in human peripheral lymphocytes from three healthy donors exposed in vitro to different concentrations of gliphosate, vinclozolin, atrazine, and DPX-E9636. The pesticides gliphosate, vinclozolin, and atrazine have been studied in a broad range of genetic tests with predominantly conflicting or negative results, whereas little is known about the genotoxicity of DPX-E9636. In our experimental conditions, each chemical compound tested produced a dose-related increase in the percent of aberrant cells and an increase of SCE/cell. Furthermore, at the highest concentrations of vinclozolin, atrazine, and DPX-E9636, we observed a significant reduction of the mitotic index. The increase of G6PD activity in exposed lymphocyte cultures strongly indicated an induction of a pro-oxidant state of the cells as an initial response to pesticide exposure.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::2fdb244273f251a67549f1703255b969 https://pubmed.ncbi.nlm.nih.gov/9707097 Zobrazit plný text záznamu Plný text