| Autor: |
N, Gomes, M, Berard, J, Vassy, N, Peyri, C, Legrand, F, Fauvel-Lafeve |
| Rok vydání: |
2002 |
| Předmět: |
|
| Zdroj: |
Biorheology. 40(1-3) |
| ISSN: |
0006-355X |
| Popis: |
The adhesion of breast adenocarcinoma cells (MDA-MB-231) to human umbilical vein endothelial cells (HUVEC) was studied in whole blood and under varying flow conditions. This study was done on HUVEC either kept under static conditions or pre-conditioned in flow for 2 hours at a shear stress of 5 or 13 dyn/cm(2). Coverslips coated by HUVEC were placed in a parallel plate perfusion chamber and perfused at a shear rate of 300 or 1500 sec(-1) with heparin-anticoagulated blood containing 111In labelled MDA-MB-231 cells. We report here the optimal conditions for studying the adhesion of MDA-MB-231 to endothelial cells under shear constraints corresponding to those observed into small and medium sized arteries. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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