Demonstration of keratin filaments in thymic nurse cells (TNC) and alloreactivity of TNC-T cell population

Autor:	D D, Vakharia
Rok vydání:	1983
Předmět:	Mice Inbred BALB C T-Lymphocytes Lymphocyte Cooperation Epithelial Cells Thymus Gland Lymphocyte Activation Epithelium Mice Mice Inbred AKR Pregnancy Mice Inbred CBA Animals Keratins Female Lymph Nodes Lymphocyte Culture Test Mixed Cytoskeleton
Zdroj:	Thymus. 5(1)
ISSN:	0165-6090
Popis:	In vitro cultured epithelial reticular cells from foetal thymic explants and thymic nurse cells (TNC) demonstrate the presence of keratin filaments in the cytoplasm. Keratin-positive cells of cultured thymic epithelium are heterogeneous in size and shape as are TNC. T cells inside the nurse cells (TNC-T) ooze out after 18 h of incubation at 37 degrees C. The alloreactivity of these T cells was compared with that of thymocytes and lymph node (LN) cells in a miniaturised mixed lymphocyte reaction using hanging drops set up in Terasaki plates. AKR TNC-T cells and LN cells showed significant proliferative response when stimulated by BALB/c LN cells. No significant proliferative response was shown by non-TNC thymocytes. The data suggest that TNC harbour a mature sample of the T-cell population as judged by alloreactivity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::2e39e1f44c5763cb357a183f11796d31 https://pubmed.ncbi.nlm.nih.gov/6189269 Zobrazit plný text záznamu