| Autor: |
Iu A, Ovchinnikov, N G, Abdulaev, M Iu, Feĭgina, I D, Artamonov, A S, Bogachuk |
| Rok vydání: |
1983 |
| Předmět: |
|
| Zdroj: |
Bioorganicheskaia khimiia. 9(10) |
| ISSN: |
0132-3423 |
| Popis: |
Tryptic hydrolysis of apomembranes, BNPS-skatole cleavage of carboxymethylated rhodopsin and thermolytic digestion of native membranes were carried out to obtain the peptides necessary for the polypeptide chain reconstruction. Gel-filtration on Bio-Gel P-30 in 80% formic acid, ion-exchange and reversed-phase high performance liquid chromatography were used for the peptide isolation. A comparison of rhodopsin hydrophobicity profile with the accessibility of the polypeptide chain in native photoreceptor membranes for proteases allowed to distinguish seven alpha-helical segments and propose a model for arrangement of the protein molecule in the membrane. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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