| Autor: |
Xiao-Li, Zou, Hong-Yan, Zeng, Yuan-Qian, Li, Tang-Lin, Mei, Bo, Jiang |
| Rok vydání: |
2009 |
| Předmět: |
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| Zdroj: |
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition. 40(5) |
| ISSN: |
1672-173X |
| Popis: |
To establish a high performance anion chromatography method for the determination of adenosine in food.Adenosine in the food sample was extracted with Acetonitrile-water, and separated with Amino PAC PA10 Amino acid-separation column, with 0.25 mol/L NaOH as the mobile phase. A pulse ampere detector with Au as working electrode was used throughout the experiment.A good linear result was produced for adenosine in the range of 1.00-20.00 microg/mL. The inner-day RSD of peak area and retention time for the standard adenosine solution were 4.97% and 0.47%, respectively. The intra-day RSD of peak area and retention time for the standard adenosine solution were 6.08% and 0.52%, respectively. The proposed method was applied to food and satisfactory results were obtained, with and average recoveries of 93.3%-106.7% and 8.86% of RSD.The high performance anion exchange chromatography method is a simple, accurate, and reliable method for measuring adenosine in food, which can be completed within 10 minutes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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