Role of Qa-1(b)-binding receptors in the specificity of developing NK cells
| Autor: | M, Salcedo, F, Colucci, P J, Dyson, L A, Cotterill, F A, Lemonnier, P, Kourilsky, J P, Di Santo, H G, Ljunggren, J P, Abastado |
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| Rok vydání: | 2000 |
| Předmět: |
Cytotoxicity
Immunologic Aging Tetrahydronaphthalenes Ligands Mice L Cells Animals Antigens Ly Lectins C-Type Amino Acid Sequence Receptors Immunologic Cells Cultured Mice Knockout Histocompatibility Antigens Class I H-2 Antigens Cell Differentiation Adoptive Transfer DNA-Binding Proteins Killer Cells Natural Mice Inbred C57BL Self Tolerance Animals Newborn Interleukin-2 Peptides beta 2-Microglobulin Spleen Receptors NK Cell Lectin-Like |
| Zdroj: | European journal of immunology. 30(4) |
| ISSN: | 0014-2980 |
| Popis: | NK cells acquire the ability to recognize MHC class I molecules during development. Studies with Qa-1(b) tetramers (Qa-1 tetramers) showed that nearly all NK1.1(+) cells from newborn C57BL/6 mice express Qa-1-binding receptors. Cytotoxic activity of these cells is fully inhibited by Qa-1 ligands on target cells. In contrast, neither receptors for H-2K(b) nor H-2D(b) were observed on NK1.1(+) cells from newborn mice. After birth, frequencies of Qa-1 tetramer(+)/ NK1.1(+) cells gradually decrease as the number of Ly49(+) /NK1.1(+) cells increases. Cell transfer studies showed that Qa-1 tetramer(+) cells from newborn mice do not lose expression of Qa-1 receptors, but that they further acquire expression of Ly49 molecules. Acquisition of Qa-1-binding receptors appears largely independent of host MHC class I molecules, as observed in studies using beta2-microglobulin-deficient (beta2m(-/-)) mice as well as K(b)/ D(b-/-) and K(b)/D(b)/beta2m(-/-) mice. The present results suggest that Qa-1-binding receptors play an important role in the specificity of developing NK cells, and suggest that these cells rely mainly on inhibitory receptors specific for non-classical MHC class I molecules to maintain self tolerance during the first weeks of life. |
| Databáze: | OpenAIRE |
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