| Autor: |
G, Kolios, K L, Wright, J D, Linehan, D A, Robertson, J, Westwick |
| Rok vydání: |
2000 |
| Předmět: |
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| Zdroj: |
Hepato-gastroenterology. 47(33) |
| ISSN: |
0172-6390 |
| Popis: |
Nitric oxide synthesis is increased in rectal biopsies from patients with ulcerative colitis and colonic epithelial cells are considered to be a major source of nitric oxide in intestinal inflammation.Human colonic biopsies from normal bowel mucosa and colonic epithelial cell line HT-29 were cultured in the presence of the inflammatory cytokines IL-1 alpha + TNF-alpha + IFN-alpha added after 1 hour pretreatment with vehicle or Interleukin-13. Nitrite levels were determined at 30 hours in culture supernatants by a fluorometric assay.Unstimulated human colonic biopsies and HT-29 cells produced a basal amount of nitrite. Stimulation with IL-1 alpha + TNF-alpha + IFN-alpha induced a significant (P0.001) increase of nitrite generation by both human colonic biopsies and HT-29 cells. The presence of Interleukin-13 produced a significant (P0.001) suppression of the cytokine-induced nitrite generation from both colonic biopsies and HT-29 cells.Nitric oxide generation in human colonic mucosa is susceptible to manipulation by proinflammatory cytokines. Interleukin-13 has an inhibitory effect on cytokine induced nitrite production in colonic mucosa and could play an anti-inflammatory role in intestinal inflammation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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