Distribution of bradykinin B2 receptors in target cells of kinin action. Visualization of the receptor protein in A431 cells, neutrophils and kidney sections
| Autor: | M, Haasemann, C D, Figueroa, L, Henderson, S, Grigoriev, S, Abd Alla, C B, Gonzalez, I, Dunia, J, Hoebeke, K, Jarnagin, J, Cartaud |
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| Rok vydání: | 1994 |
| Předmět: |
Receptor
Bradykinin B2 Neutrophils Receptors Bradykinin Molecular Sequence Data Fluorescent Antibody Technique Sequence Homology Kinins Kidney Rats Immunoenzyme Techniques Rats Sprague-Dawley Species Specificity Culture Techniques Tumor Cells Cultured Animals Autoradiography Humans Amino Acid Sequence Binding Sites Antibody Cells Cultured |
| Zdroj: | Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas. 27(8) |
| ISSN: | 0100-879X |
| Popis: | Peptides corresponding to sequences derived from predicted extra- and intracellular loops of the rat bradykinin receptor were analyzed for interspecies homology as well as for matches within the present dataset of protein sequences to provide a theoretical basis for the specific recognition of the native cognate protein by antibodies raised against these antigens. Application of polyclonal antibodies raised against the selected peptides allowed the immunocytochemical localization of the native receptor protein in cells of rat and human origin. The detection of the molecule was achieved by different immunohisto- and immunocytochemical methods in combination with light, fluorescence, confocal optical laser and electron microscopy. These results were compared to localization studies by autoradiography. Distribution and subcellular localization were determined in human neutrophils, human epithelial carcinoma cells (A431) and in rat kidney tissue. |
| Databáze: | OpenAIRE |
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