| Autor: |
E E, Brevnova, D G, Kozlov, B D, Efremov, S V, Benevolensky |
| Rok vydání: |
1999 |
| Předmět: |
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| Zdroj: |
Biotechnology and bioengineering. 60(4) |
| ISSN: |
0006-3592 |
| Popis: |
The gene encoding inulase of the yeast Kluyveromyces marxianus (INU1Km) was cloned and expressed in the inulin-negative yeast Saccharomyces cerevisiae. Cells of S. cerevisiae transformed with the INU1Km gene have acquired extracellular inulase activity and were able to grow in the medium with inulin as a sole carbon source. The S. cerevisiae strain was constructed that is capable of heterologous expression of secreted K. marxianus inulase and is defective in fructose uptake due to null-mutations of the hexokinase structural genes HXK1 and HXK2. When grown in inulin-containing media, this strain is capable of accumulating at least 10% glucose-free fructose in the culture liquid. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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