| Autor: |
V, Sanchez, S, Gimenez, J, Haensler, C, Geoffroy, B, Rokbi, D, Seguin, L, Lissolo, B, Harris, F, Rizvi, H, Kleanthous, T, Monath, M, Cadoz, B, Guy |
| Rok vydání: |
2001 |
| Předmět: |
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| Zdroj: |
FEMS immunology and medical microbiology. 30(2) |
| ISSN: |
0928-8244 |
| Popis: |
The ability to induce a protective response against Helicobacter pylori infection has been investigated by systemic immunization of mice with urease formulated with the cationic lipid DC Chol. This compound acts both as a formulating agent and as an adjuvant and induces a balanced Th1/Th2 response shown to be more effective for protection in our previous studies. Urease-DC Chol induced a significant protection in prophylaxis but not in therapeutic immunization. The protection level was between 1.5 and 2 log reduction of bacterial density measured by quantitative culture compared to unimmunized-infected mice. In parallel, the protective efficacy of other H. pylori antigens formulated in a similar way and administered with DC Chol was tested. These antigens were tested alone or in combination in prophylactic and therapeutic regimens. Some combinations of antigens induced a better prophylactic or therapeutic activity than urease alone (0.5-1.5 log further reduction in prophylaxis and therapy respectively, P0.05). The combinations that induced the best protection were different in prophylaxis and therapy. In conclusion, DC Chol provides a convenient and efficient method to formulate different antigens even when they are present in non-compatible buffers initially. Moreover, the results obtained in protection against H. pylori with such formulations should lead the way to future clinical trials. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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