Collection of 'normal' blood repopulating cells during early hemopoietic recovery after intensive conventional chemotherapy in chronic myelogenous leukemia
| Autor: | A M, Carella, M, Podesta, F, Frassoni, M R, Raffo, N, Pollicardo, E, Pungolino, R, Vimercati, M, Sessarego, C, Parodi, C, Rabitti |
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| Rok vydání: | 1993 |
| Předmět: |
Adult
Male Time Factors Fusion Proteins bcr-abl Blood Component Transfusion Leukemia Myeloid Accelerated Phase Cell Separation Polymerase Chain Reaction Blood Transfusion Autologous Leukemia Myelogenous Chronic BCR-ABL Positive Antineoplastic Combined Chemotherapy Protocols Granulocyte Colony-Stimulating Factor Humans Immunologic Factors RNA Messenger RNA Neoplasm Etoposide Remission Induction Cytarabine Hematopoietic Stem Cell Transplantation Interferon-alpha Middle Aged Hematopoietic Stem Cells Combined Modality Therapy Recombinant Proteins Treatment Outcome Leukemia Myeloid Chronic-Phase Female Idarubicin |
| Zdroj: | Bone marrow transplantation. 12(3) |
| ISSN: | 0268-3369 |
| Popis: | Twenty-five patients with CML (chronic phase (CP): 15 patients; accelerated phase (AP): 10 patients) at a median of 40 months after diagnosis and ineligible for allogeneic BMT, received an intensive chemotherapy regimen consisting of idarubicin, intermediate-dose ara-C and etoposide (ICE protocol). All patients had previously received alpha-interferon and only two patients had had partial cytogenetic response. During recovery from chemotherapy-induced aplasia, blood progenitors cells (BPC) were harvested by leukapheresis. All metaphases were found to be Ph-negative in the collection of 12 of 25 (48%) patients (CP: 9 of 15 (60%), AP: 3 of 10 (30%)) and a decrease of50% Ph-positive metaphases was seen in an additional five (CP: 4 patients; AP: 1 patient). The percentage of complete Ph-disappearance was 66% in patients receiving this procedure within the first 2 years of diagnosis and 30% in those treated after the second year of diagnosis. So far, the Ph-negative collections have been used in 9 patients (CP: 8 patients; AP: 1 patient) as autograft after conditioning with total body irradiation/etoposide/CY. Seven of 9 patients engrafted and 5 are alive and well, Ph-negative at 2+, 3+, 6+, 10+ and 18+ months. |
| Databáze: | OpenAIRE |
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