Sialyltransferase activity in normal and atherosclerotic human aorta intima

Autor:	E V, Gracheva, N N, Samovilova, N K, Golovanova, O P, Il'inskaya, E M, Tararak, N V, Prokazova
Rok vydání:	2001
Předmět:	Adult Male Membrane Glycoproteins Arteriosclerosis In Vitro Techniques Sialyltransferases Enzyme Activation Kinetics Reference Values Humans Female Tunica Intima Aorta Aged
Zdroj:	Biochemistry. Biokhimiia. 66(4)
ISSN:	0006-2979
Popis:	Sialyltransferase activity has been determined in Golgi membrane fractions isolated from atherosclerotic and normal intima of human aorta by measuring the transfer of N-acetylneuraminic acid (NeuAc) from CMP-NeuAc to asialofetuin. The asialofetuin-sialyltransferase activity was found to be twofold higher in the atherosclerotic intima than in the normal intima. The mean value of the apparent Michaelis constant (Km) for the sialylating enzyme in both tissues did not differ and was 57 microM. In contrast, the maximal velocity (Vmax) was 2-fold higher for the atherosclerotic intima than for the normal intima. These results suggest that expression of asialofetuin-sialyltransferases of the aortal intima may be increased in atherosclerosis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::2c53844bed632b335cb9bbf803c7ba58 https://pubmed.ncbi.nlm.nih.gov/11403646 Zobrazit plný text záznamu Full text from SpringerLink