| Popis: |
The re-aggregation of dispersed islet cells from non-inbred ob/ob-mice was studied by light and electron microscopy. After 3 days of culture, spontaneously formed aggregates with more than 95% beta-cells were up to 0.5 mm in diameter and exhibited a high degree of viability on dye exclusion tests. In comparison with cultures at 1 or 3 mM D-glucose, or 1 mM D-glucose in combination with 19 mM 3-0-methyl-D-glucose, aggregates formed in 20 mM D-glucose were more closely packed, had a smoother circumference with elongated peripheral beta-cells, and exhibited well developed micro-villi in localized intercellular widenings. A stereological analysis of electron micrographs showed that beta-cells aggregated at 20 mM D-glucose exhibited the same individual profile area but a significantly lower form factor, and a significant reduction in granule volume density as compared with aggregates at 3 mM D-glucose. It is concluded that D-glucose has morphogenetic effects on both the cellular and the micro-anatomical level of pseudo-islet structure in culture. |
