Western immunoblotting as a method for the detection of African horse sickness virus protein-specific antibodies: differentiation between infected and vaccinated horses

Autor:	S I, Bougrine, O F, Fihri, M M, Fehri
Rok vydání:	1998
Předmět:	Viral Proteins Vaccines Inactivated African Horse Sickness Blotting Western Chlorocebus aethiops Vaccination African Horse Sickness Virus Animals Viral Vaccines Horses Antibodies Viral Vaccines Attenuated Vero Cells
Zdroj:	Archives of virology. Supplementum. 14
ISSN:	0939-1983
Popis:	A Western immunoblotting procedure has been developed for the detection of African horse sickness virus (AHSV) protein-specific antibody responses. This assay readily identifies antibodies specific for at least 4 distinct, AHSV proteins, including VP5, NS1, NS2 and NS3/NS3a. By using the AHSV non-structural proteins as 'markers', the Western blotting procedure could be employed to provide a reliable means of discriminating between animals vaccinated with a purified, inactivated AHSV vaccine and those either naturally infected or vaccinated with a live, attenuated AHSV vaccine.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::2c2cc417eac2f41f4ae3928e529e1a47 https://pubmed.ncbi.nlm.nih.gov/9785518 Zobrazit plný text záznamu