| Autor: |
Anatoly, Soloviev, Irina, Ivanova, Vadym, Sydorenko, Khrystyna, Sukhanova, Mariia, Melnyk, Dariia, Dryn, Alexander, Zholos |
| Rok vydání: |
2022 |
| Zdroj: |
Acta physiologica (Oxford, England). |
| ISSN: |
1748-1716 |
| Popis: |
Gold nanoparticles are widely used for biomedical applications, but the precise molecular mechanism of their interaction with cellular structures is still unclear. Assuming that intracellular calcium fluctuations associated with surface plasmon-induced calcium entry could modulate the activity of potassium channels, we studied the effect of 5 nm gold nanoparticles on calcium-dependent potassium channels and associated calcium signaling in freshly isolated rat pulmonary artery smooth muscle cells and cultured hippocampal neurons.Outward potassium currents were recorded using patch-clamp techniques. Changes in intracellular calcium concentration were measured using the high affinity CaIn pulmonary artery smooth muscle cells, plasmonic gold nanoparticles increased the amplitude of currents via large-conductance CaWe conclude that fluctuations in intracellular calcium can modulate plasmonic gold nanoparticles-induced gating of BK |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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