| Autor: |
Eva M, Clavane, Hannah A, Taylor, Richard M, Cubbon, Paul J, Meakin |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 2441 |
| ISSN: |
1940-6029 |
| Popis: |
The fibrin gel angiogenesis bead assay provides a controlled in vitro setting for observing endothelial angiogenic sprouting in response to modified variables. Endothelial cells are coated onto microcarriers and embedded into a fibrin clot containing necessary growth factors. Following a 24-h incubation, endothelial sprouts are imaged using a light microscope. This method is useful for rapidly and affordably investigating the effects of genetic or chemical manipulation to endothelial function. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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