Identification of membrane proteome of
| Autor: | Juliana Santana, de Curcio, Marielle Garcia, Silva, Mirelle Garcia, Silva Bailão, Sônia Nair, Báo, Luciana, Casaletti, Alexandre Mello, Bailão, Célia Maria, de Almeida Soares |
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| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Future Science OA |
| ISSN: | 2056-5623 |
| Popis: | Aim: During infection development in the host, Paracoccidioides spp. faces the deprivation of micronutrients, a mechanism called nutritional immunity. This condition induces the remodeling of proteins present in different metabolic pathways. Therefore, we attempted to identify membrane proteins and their regulation by zinc in Paracoccidioides lutzii. Materials & methods: Membranes enriched fraction of yeast cells of P. lutzii were isolated, purified and identified by 2D LC–MS/MS detection and database search. Results & conclusion: Zinc deprivation suppressed the expression of membrane proteins such as glycoproteins, those involved in cell wall synthesis and those related to oxidative phosphorylation. This is the first study describing membrane proteins and the effect of zinc deficiency in their regulation in one member of the genus Paracoccidioides. Lay abstract The methodology of protein identification allows the characterization of biological processes performed by those molecules. Therefore, we performed a membrane proteomic analysis of Paracoccidioides lutzii and further evaluated the responses of the fungus to zinc deprivation. The results obtained in the work allowed the characterization of membrane proteins present in organelles that are related to different cellular functions. Zinc deprivation changes processes related to cellular physiology and metabolism. These results help us to understand the process of pathogen–host interaction, since zinc deprivation is a condition present during infection. |
| Databáze: | OpenAIRE |
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