Role of inflammatory mediators in human endometrium during progesterone withdrawal and early pregnancy
| Autor: | H O, Critchley, R L, Jones, R G, Lea, T A, Drudy, R W, Kelly, A R, Williams, D T, Baird |
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| Rok vydání: | 1999 |
| Předmět: |
Estradiol
Reverse Transcriptase Polymerase Chain Reaction Interleukin-8 Membrane Proteins Enzyme-Linked Immunosorbent Assay Dinoprost Immunohistochemistry Isoenzymes Endometrium Cyclooxygenase 2 Pregnancy Prostaglandin-Endoperoxide Synthases Leukocytes Humans Female RNA Messenger Inflammation Mediators Chemokine CCL2 Progesterone |
| Zdroj: | The Journal of clinical endocrinology and metabolism. 84(1) |
| ISSN: | 0021-972X |
| Popis: | The role of progesterone (P4) in the regulation of inflammatory mediators interleukin-8 (IL-8), monocyte chemoattractant protein-1, and cyclooxygenase-2 (COX-2) and in the recruitment of leukocyte subpopulations in the endometrium has been examined, by employing a model of P4 withdrawal and maintenance in vivo. Messenger RNA and protein expression have been investigated in endometrial biopsies: 1) during the midsecretory phase (LH+8 to 10); during the maintained luteal phase (P4 administered vaginally for 4 days from LH+8) and biopsies collected 2) 24 h and 3) 48 h post withdrawal of P4; and 4) during pseudo pregnancy (lifespan of corpus luteum extended by 7 days with CG; (decidua collected from women with 5) an ectopic gestation and 6) from women undergoing first-trimester termination of pregnancy). CD56+ large granular lymphocytes remain the major leukocyte subtype, both 24 and 48 h after P4 withdrawal, and in decidua (CG supported or ectopic). Higher numbers (P0.05) of macrophages (CD68+) were present in endometrium 48 h post P4 withdrawal and in pseudo pregnant endometrium, compared with normal decidua. Significantly more macrophages (P0.01) were present in decidua from an ectopic pregnancy. A significant elevation of IL-8 (P0.01) and COX-2 (P0.05) messenger RNA was detected 48 h post P4 withdrawal. Evidence is provided for up-regulation of IL-8 and COX-2 in response to P4 withdrawal. |
| Databáze: | OpenAIRE |
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