Direct culture-independent sequence typing of

Autor: Catherine E, May, Alan J, Guthrie, Martin L, Schulman
Rok vydání: 2019
Předmět:
Zdroj: J Vet Diagn Invest
ISSN: 1943-4936
Popis: We report herein the use of crude extracts obtained from samples of Taylorella equigenitalis–infected horses for the purpose of multi-locus sequence typing (MLST). Samples (n = 36) were collected from horses in South Africa from 1996 to 2017: 34 from genital swabs (stored at −20°C for 2–3 y) and 2 from cryopreserved raw semen aliquots (stored at −70°C for 18 y) prior to assay. The MLST assay showed a single sequence type (ST), designated ST4, that supported a point introduction and thus a common source for the South African outbreak of contagious equine metritis.
Databáze: OpenAIRE