[Interaction of short oligonucleotide derivatives with nucleic acids. V. Ligation of short oligonucleotides in tandem on a complimentary DNA template]

Autor:	D V, Pyshnyĭ, A A, Krivenko, S G, Lokhov, E M, Ivanova, G M, Dymshits, V F, Zarytova
Rok vydání:	1998
Předmět:	DNA Complementary DNA Ligases Genes Reporter Oligonucleotides Autoradiography Nucleic Acid Hybridization Electrophoresis Polyacrylamide Gel Templates Genetic Nucleic Acid Denaturation Fluorescent Dyes Repetitive Sequences Nucleic Acid
Zdroj:	Bioorganicheskaia khimiia. 24(1)
ISSN:	0132-3423
Popis:	A tetranucleotide was highly specifically and quantitatively ligated with a pair of flanking octanucleotides carrying both radioactive and nonradioactive reporter groups. The sequence of the ligation of oligonucleotide components in a tandem on a complementary template was studied. The first stage was found to be the enzyme-catalyzed activation of the phosphate group of octanucleotide, a tandem component that possesses a higher hybridization capacity than the tetramer. It is shown that the introduction of terminal reporter groups into octanucleotides does not decrease the efficiency of their tandem ligation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::2b85f1acdf9cfd2f31860d18b77f6edd https://pubmed.ncbi.nlm.nih.gov/9551198 Zobrazit plný text záznamu