Mycoplasma pneumoniae attachment to WiDr cell cultures: competitive inhibition assays

Autor:	Chandler, D. K., Barile, M. F.
Jazyk:	angličtina
Rok vydání:	1983
Předmět:	Binding Sites Erythrocytes Carcinoma Adhesiveness digestive system Binding Competitive Models Biological respiratory tract diseases Cell Line Mycoplasma pneumoniae Gangliosides Intestinal Neoplasms Humans Research Article Glycoproteins
Zdroj:	The Yale Journal of Biology and Medicine
ISSN:	1551-4056 0044-0086
Popis:	Attachment of radiolabeled M. pneumoniae to human WiDr cell culture monolayers was dependent on the WiDr cell density and the concentration of M. pneumoniae. Saturation of confluent monolayers grown on 5 mm coverslips was attained with only 40 micrograms of M. pneumoniae protein. Preincubating the WiDr monolayers with unlabeled M. pneumoniae or with a protein-rich extract prepared from M. pneumoniae inhibited subsequent attachment of radiolabeled organisms. Attachment inhibition by the M. pneumoniae extract provided a quantitative assay for mycoplasmal binding components. Treatment of radiolabeled M. pneumoniae with orosomucoid, ceruloplasmin, and gangliosides inhibited attachment to WiDr cells. These sialoglycoconjugates may be structural analogues of the target cell receptor.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::2af4eb6e3398a010e5a47cf5717b5396 http://europepmc.org/articles/PMC2590571 Zobrazit plný text záznamu