| Autor: |
M, Urtizberea, M, Latour, L, Edelman, J, Carcagne, J, Armand, J M, Scherrmann |
| Rok vydání: |
1990 |
| Předmět: |
|
| Zdroj: |
Developments in biological standardization. 71 |
| ISSN: |
0301-5149 |
| Popis: |
A monoclonal antibody specific to digitoxin was developed and Fab fragments were prepared using the conventional papain method. The affinity constant was determined as 10(9)M-1 and the cross reactivity with digoxin was 2%. The Fab fragments were used for the reversal of acute digitoxin poisoning in rabbits (100% mortality). Fab fragments were administered over a 40 or 80 minute period just after digitoxin infusion. Rabbits treated with specific Fab fragments were protected by a bistoichiometrical dose. Evaluation of plasma digitoxin confirmed that the specific Fab fragments were able to extract and sequestrate digitoxin. The digitoxin-Fab fragment complexes were removed by renal excretion. Our data provide evidence of the benefit of monoclonal Fab fragments specific for digitoxin for the reversal of acute digitoxin poisoning. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
