Activated in prostate cancer: a PDZ domain-containing protein highly expressed in human primary prostate tumors
| Autor: | H, Chaib, M A, Rubin, N R, Mucci, L, Li, Taylor JMG, M L, Day, J S, Rhim, J A, Macoska |
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| Rok vydání: | 2001 |
| Předmět: |
Male
Binding Sites Base Sequence Gene Expression Profiling Molecular Sequence Data Prostatic Neoplasms Neoplasm Proteins Protein Structure Tertiary Up-Regulation Gene Expression Regulation Neoplastic Tumor Cells Cultured Humans RNA Messenger Cell Adhesion Molecules Adaptor Proteins Signal Transducing Oligonucleotide Array Sequence Analysis |
| Zdroj: | Cancer research. 61(6) |
| ISSN: | 0008-5472 |
| Popis: | Critical events in prostate tumorigenesis and metastasis likely include the abnormal activation and expression of specific genes. Using RNA expression profiling techniques, we have identified a transcript originating from the activated in prostate cancer (AIPC) gene, the expression of which is preferentially up-regulated in several cultured prostate tumor cell lines and human primary prostate tumors. Sequence analysis revealed that the AIPC protein encodes six PDZ domains, which are protein-protein binding domains likely involved in protein clustering and scaffolding. Immunohistochemical analysis of a tissue microarray comprising 158 tumor, 18 high-grade prostatic intraepithelial neoplasia, and 91 normal prostate specimens with an anti-AIPC antibody demonstrated abundant AIPC protein expression in 75% of tumors, 83% of prostatic intraepithelial neoplasia lesions, and 3% of normal tissues (P0.0001). These data suggest that the accumulation of AIPC protein may be closely associated with the initiation or early promotion of prostate tumorigenesis. |
| Databáze: | OpenAIRE |
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