SUMOylation does not affect cardiac troponin I stability but alters indirectly the development of force in response to Ca

Autor:	Bracy, Fertig, Jiayue, Ling, Edgar E, Nollet, Sara, Dobi, Tara, Busiau, Kiyotake, Ishikawa, Kelly, Yamada, Ahyoung, Lee, Changwon, Kho, Lauren, Wills, Amy J, Tibbo, Mark, Scott, Kirsten, Grant, Kenneth S, Campbell, Emma J, Birks, Niall, MacQuaide, Roger, Hajjar, Godfrey L, Smith, Jolanda, van der Velden, George S, Baillie
Rok vydání:	2022
Předmět:	Myofibrils Lysine Troponin I Humans Sumoylation Calcium Phosphorylation
Zdroj:	The FEBS journal. 289(20)
ISSN:	1742-4658
Popis:	Post-translational modification of the myofilament protein troponin I by phosphorylation is known to trigger functional changes that support enhanced contraction and relaxation of the heart. We report for the first time that human troponin I can also be modified by SUMOylation at lysine 177. Functionally, TnI SUMOylation is not a factor in the development of passive and maximal force generation in response to calcium, however this modification seems to act indirectly by preventing SUMOylation of other myofilament proteins to alter calcium sensitivity and cooperativity of myofilaments. Utilising a novel, custom SUMO site-specific antibody that recognises only the SUMOylated form of troponin I, we verify that this modification occurs in human heart and that it is upregulated during disease.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::2a963182ab602f3d02da63c30a7daf88 https://pubmed.ncbi.nlm.nih.gov/35633070 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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